Table 1 RNA integrity and quantity of microdissected samples.

From: An Optimised Protocol Harnessing Laser Capture Microdissection for Transcriptomic Analysis on Matched Primary and Metastatic Colorectal Tumours

SN

Sample ID

RIN

Total RNA input 5 µl (ng)

1

Pat 1 Colonic Mucosa Stroma

7.9

4.82

2

Pat 1 Colonic Mucosa Epithelial

5

59.22

3

Pat 1 Primary CRC Stroma

7

47.20

4

Pat 1 Primary CRC Tumour

8

41.60

5

Pat 1 Krukenberg Stroma

7.9

28.85

6

Pat 1 Krukenberg Tumour

7.5

57.00

7

Pat 2 Colonic Mucosa Stroma

5

3.80

8

Pat 2 Colonic Mucosa Epithelial

3.1

16.17

9

Pat 2 Primary CRC Stroma

5.2

11.97

10

Pat 2 Primary CRC Tumour

6.3

24.08

11

Pat 2 Right Krukenberg Stroma

4.5

12.55

12

Pat 2 Right Krukenberg Tumour

7.1

28.96

13

Pat 2 Left Krukenberg Stroma

5.9

10.30

14

Pat 2 Left Krukenberg Tumour

7.1

20.76

15

Pat 3 Primary CRC Stroma

3

20.05

16

Pat 3 Primary CRC Tumour

4.2

10.06

17

Pat 3 Krukenberg Stroma

5

56.04

18

Pat 3 Krukenberg Tumour

4.1

81.14

19

Pat 4 Krukenberg Stroma

3

17.08

20

Pat 4 Krukenberg Tumour

3.7

23.87

 

Average

5.5

28.8

 

S.D.

1.7

21.3