Figure 1 | Scientific Reports

Figure 1

From: A higher throughput assay for quantification of melphalan-induced DNA damage in peripheral blood mononuclear cells

Figure 1

Quantitative PCR-block (QPCR-block) is a well-validated sequence-based approach based on the ability of DNA lesions to block DNA polymerase and stall amplification. Increasing numbers of bulky DNA adducts and interstrand crosslinks (ICL), formed by alkylating agents such as melphalan, in the DNA template proportionally decrease the amplification of a target sequence. Lower amplification reflects higher DNA damage. The amount of target double stranded DNA (dsDNA) amplicon can be then quantified and inhibition of PCR relative to untreated control template is reported as % QPCR-block.

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