Figure 3
Detection of QPCR-block following exposure of (A) naked gDNA (7 µg) and (B) intact PBMC (1 × 106 cells) to increasing concentrations of melphalan for 1 h at 37 °C. The 1.6 kb TP53 amplicon PCR products, analysed in triplicate, were quantified using fluorescence spectrophotometry. Data are shown as the mean ± standard error across three independent experiments. Error bars may be smaller than the symbols. Data are shown relative to untreated control (Y1 axis), and also as directly quantified amplicon (Y2 axis). IC50 curve fits with 95% CI (dotted lines) are shown.
