Figure 4 | Scientific Reports

Figure 4

From: A higher throughput assay for quantification of melphalan-induced DNA damage in peripheral blood mononuclear cells

Figure 4

Reproducible detection of melphalan-induced QPCR-block from PBMC collected on three independent occasions from each of three individual PBMC donors (MEL004, MEL005, MEL009). PCR products were quantified using fluorescence spectrophotometry. (A) IC50 curve fits with 95% CI (dotted lines). (B) Estimation of the number of melphalan-induced DNA adducts per kilobase, based on a Poisson equation. Data are mean ± SD of three experimental repeats, each experiment had n = 4 technical repeats at each concentration tested.

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