Figure 6

Production of ROS and activation of Caspase3/7 by compound 1 and 2 in PCa cells. (A) The fold change in ROS in PC-3 and DU-145 cells treated with compound 1, 2 and H₂O₂. H2DCFDA labelled cells was used to measure the ROS production and its fluorescence signal was recorded using 96 well plate reader. The fold change of ROS was calculated using fluorescence intensities of the untreated control. (B) The fold change in Caspase 3/7 in PC-3 and DU-145 cells treated with compound 1, 2, and Na₃VO₄. Biological and the technical replicates were maintained to assess the significance of the results, with mean ± S.D. *P < 0.05, versus control, n = 3.