Table 1 PCR primers sequences and expected amplicon size.
Gene | protein | Forward primers (5′ → 3′) | Reverse primers (5′ → 3′) | Expected size (bp) |
|---|---|---|---|---|
ETS1 | v-ets erythroblastosis virus E26 oncogene homolog 1 | CTGCGCCCTGGGTAAAGA | CCCATAAGATGTCCCCAACAA | 65 |
HPRT1 | Hprt1 | TTGCTGACCTGCTGGATTAC | TATGTCCCCTGTTGACTGGT | 119 |
PPIA | Peptidylprolyl Isomerase A (PPIA), Cyclophilin A | ACCGCCGAGGAAAACCGTGTA | TGCTGTCTTTGGGACCTTGTCTGC | 129 |
SCN5A | NaV1.5 | CACGCGTTCACTTTCCTTC | CATCAGCCAGCTTCTTCACA | 208 |
SIK1 | Salt-Inducible Kinase 1 (SIK1) | TCCAGACCATCTTGGGGCAG | AAGGGGAAGGGGTTTTGTGTTG | 86 |
SLC9A1 | Sodium-proton Exchanger type 1 (NHE1) | TCTTCACCGTCTTTGTGCAG | AAGGTGGTCCAGGAACTGTG | 125 |
SNAI1 | Snai1 | AATCCAGAGTTTACCTTCCAGCA | TCCCAGATGAGCATTGGCAG | 110 |
TWIST1 | Twist1 | CGGGAGTCCGCAGTCTTA | GCTTGAGGGTCTGAATCTTG | 161 |
ZEB1 | Zeb1 | TGCACTGAGTGTGGAAAAGC | TGGTGATGCTGAAAGAGACG | 237 |
