Figure 2
From: Assessing average somatic CAG repeat instability at the protein level
PolyQ length-dependent effect on mHTT detection is also observed with full length mHTT from HD-KI mice. Homogenates from striatum of 6 months old HD-KI mice with 50, 80, 111, 140 and 175 CAG repeats were analyzed for detection of mHTT with two different capture Abs (EPR5526 and D7F7) and MW1 detection Ab. MSD signals were normalized by the amount of mHTT quantified by WB as shown in Supplementary Fig. S4. Mean values ± SD (1 σ) of n = 3 mice per group are shown.
