Figure 3
From: Assessing average somatic CAG repeat instability at the protein level
Method for HTT polyQ length quantification. HTT proteins exhibit a mosaicism of polyQ lengths in biological tissue prone to CAG repeat instability. To quantify average polyQ length in HTT proteins, the biological sample is quantified twice by sandwich ELISA-based assay with two pairs of Abs: one that includes a detection Ab that does not target the polyQ tract (a) to quantify total HTT (b) and another one that has a polyQ targeting detection Ab (c). This information is used in a mathematical model to determine the average polyQ length in HTT proteins (d) when samples are tested in the linear dynamic range.
