Table 2 Quantification of the same average polyQ length obtained by different protein mixings with MW1 detection Ab.
From: Assessing average somatic CAG repeat instability at the protein level
Theoretical Average Q length | Fraction of GST-FLAG-HTTexon1 mixed together | Average Q length experimentally determined | |||||
|---|---|---|---|---|---|---|---|
Q25 | Q38 | Q48 | Q55 | Q72 | Mean ± SD (Cv %) | %RE | |
48a | 18% | 21% | 21% | 20% | 20% | 51.75 ± 1.75 (3.37) | 7.90 |
48b | 9% | 20% | 33% | 30% | 8% | 50.71 ± 0.22 (0.44) | 5.75 |
48c | 12% | 34% | 18% | 15% | 21% | 50.28 ± 1.68 (3.34) | 4.90 |
