Figure 3 | Scientific Reports

Figure 3

From: Mutant IDH1 confers resistance to energy stress in normal biliary cells through PFKP-induced aerobic glycolysis and AMPK activation

Figure 3

The glycolytic inhibitor 2-DG or glucose restriction hampers the formation of IBOs, and mut-IBOs were resistant to the inhibition. (A) 2-DG competitively inhibits the conversion of G6P to F6P in glycolysis. (B) The number of organoids (>100 μm) at 5 d after plating in medium with or without 2DG (normalized to that in the 2DG-free medium, n = 4, 5,000 cells per group *P < 0.05). (C) Representative images. Scale bars, 250 μm. (D) The number of IBOs (>100 μm) was counted at 7 d after plating under the normal culture condition (Normal) and the low glucose condition (Low glucose), replacing advanced-DMEM/F12 (3 g/L glucose) to L0091, glucose-free DMEM/F12 instead (n = 4, 5,000 cells per group, **P < 0.01). Low glucose medium contains a little glucose derived from Wnt3a- and RSPO1-conditioned medium; the final concentration of glucose is 0.2 g/L. (E) Representative images. Scale bars, 500 μm.

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