Figure 4
Effect of GCV on the proliferation of C3H10T1/2/IFN-γ or C3H10T1/2/HSVtk/IFN-γ cells. (A) The number of C3H10T1/2/HSVtk cells treated with GCV. C3H10T1/2/HSVtk cells were cultured in media containing 0.03125, 0.0625, 0.125 or 0.25 μg/mL GCV. No treatment (white circle), 0.03125 μg/mL GCV (white square), 0.0625 μg/mL GCV (white diamond), 0.125 μg/mL GCV (white triangle), and 0.25 μg/mL GCV (black circle) are indicated. Results are expressed as the mean ± SD of four samples. A representative of two independent experiments with similar results is shown. *p < 0.05; statistically significant differences observed in comparison with no treatment group. (B) The IFN-γ concentration in the supernatant of C3H10T1/2/HSVtk/IFN-γ cells cultured in normal medium or GCV containing medium. C3H10T1/2/HSVtk/IFN-γ cells were cultured in medium containing 0.25 μg/mL GCV. The medium was replaced with fresh medium containing various concentrations of GCV, and it was changed every 24 h and collected at Day 5 and Day 9. The IFN-γ concentration in medium was measured by ELISA. No treatment (white bar) and 0.25 μg/mL GCV containing medium (black bar) are indicated. Results are expressed as the mean ± SD of four samples. A representative of two independent experiments with similar results is shown. *p < 0.05; statistically significant differences observed in comparison with no treatment group.
