Figure 6
From: 3D-printed biological cell phantom for testing 3D quantitative phase imaging systems

Holographic tomography system used in the experiments: fiber-coupled He-Ne laser (LS, λ = 632.8 nm), GM - 2D galvanometer mirrors, TL1, TL2 - tube lenses (\({f^{\prime} }_{TL1}=150\,mm\), \({f^{\prime} }_{TL2}=200\,mm\)), MO - microscope objective (100x, NA = 1.3 for illumination and 40x, NA = 1.3 for imaging), SC - sample chamber. In the illumination scanning mode the object under study is attached to the bottom coverslip, while in sample rotation (isotropic) mode, an optical fiber with the phantom printed on its end is attached to a motor and introduced between the coverslips from the side (FM - rotating fiber).