Table 1 Parameters of analysis by means of gas chromatography coupled with mass spectrometry.
From: Volatile scents of influenza A and S. pyogenes (co-)infected cells
Gas chromatograph | Agilent 7890A |
|---|---|
Desorption | thermal |
Injector temperature | 200 °C |
Mode | splitless mode (60 s) |
Column | 60 m RTX- 624 column 6% (Cyanopropylphenyl/94% dimethyl polysiloxane) |
Column thickness | 0.32 mm ID, 1.8 μm |
Carrier gas | helium |
Carrier gas flow | 1.5 ml/min |
Temperature program | 40 °C for 5 min, 8 °C/min to 120 °C for 2 min, 10 °C/min to 220 °C, 20 °C/min to 240 °C for 4.5 min |
MSD with triple axis detector | Agilent 5975C inert XL |
Ionization | electron impact ionization (EI −70 eV) |
Scan mode SIM Ions | full scan mode with mass range 35–250 amu 41, 43, 53, 57, 62, 67, 72, 91, 106, 116, 123 |
Scan rate | 2.73 scan/s |
