Figure 1 | Scientific Reports

Figure 1

From: The DNA topoisomerase II inhibitor amsacrine as a novel candidate adjuvant in a model of glaucoma filtration surgery

Figure 1

Primary screening of the candidate compounds in mTCFs. (A) Characterization of mTCFs with fibroblast-specific protein-1 immunostaining. The scale bar is 100 μm. (B) Scatter plot showing results of the primary screening to identify compounds able to inhibit cell proliferation, with an Alamar Blue assay. Ten μM MMC was used as a positive control. The fluorescence intensity after MMC treatment was normalized as 100% cell proliferation. Compounds were selected as candidates (shown by the white circles, 90 total) if they exerted an equal or stronger suppressive effect on fibroblast proliferation compared to MMC. The candidate compounds are all located below the dashed line.

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