Figure 3

Antibiofilm activity of compound 1 and amphotericin B against mature Candida biofilm. Test agents were evaluated against (a) C. albicans P60002 and (b) C. auris strain 390 adherent biofilm evaluated with the XTT assay. Adherent biofilms were treated with either compound 1 or amphotericin B (both in triplicate) at the concentrations presented, over a 24-hour period. The percent metabolic activity for each treatment was calculated relative to untreated wells. Error bars represent standard deviation values. Asterisk denotes statistical difference between compound 1 and amphotericin B relative to the negative control (untreated wells) evaluated using a two-way ANOVA, with post hoc Dunnet’s test for multiple comparisons (P < 0.05).