Table 2 Thermodynamic analysis by ITC of interactions between RCD1-RST domains and different TFs.

From: Evolutionary conservation of the intrinsic disorder-based Radical-Induced Cell Death1 hub interactome

TF

RCD1

Kd (nM)

N

ΔH (kJ/mol)

−TΔS (kJ/mol)

ΔG (kJ/mol)

Syringe

AtDREB2A(244–272)

AtRST(499–572)

16 ± 1.4

0.90 ± 0.00

−63.3 ± 0.2

18.7

−44.6

RST

AtDREB2A(244–272;L264I)

AtRST(499–572)

39 ± 7.6

0.89 ± 0.01

−53.8 ± 0.7

11.5

−42.5

RST

ANAC013(254–274)a

AtRST(499–572)

9 ± 4

0.80 ± 0.01

−45.0 ± 0.8

−0.6

−45.6

RST

ANAC013(254–274;L266I)

AtRST(499–572)

223 ± 29

0.95 ± 0.01

−42.3 ± 0.5

4.3

−38.0

RST

ANAC087(315–335)

AtRST(499–572)

1751 ± 625

0.93 ± 0.01

−15.9 ± 1.6

−16.9

−32.9

ANAC087

ANAC087(315–335)

AtRST(499–572;R551Q,R560Q)

NBb

    

RST

ANAC087(315–335)

AtRST(499–572;I563Q)

NB

    

RST

ANAC087(315–335)

AtRST(499–572;R560Q)

NB

    

ANAC087

ANAC087(315–335)

AtRST(499–572;R551Q)

NB

    

ANAC087

HvDRF1.1(255–273)

AtRST(499–572)

450 ± 169

0.95 ± 0.03

−48.7 ± 2.4

12.5

−36.2

RST

HvNAC013(176–346)

AtRST(499–572)

343 ± 180

1.03 ± 0.04

−4.5 ± 0.3

−32.5

−37.0

RST

HvDRF1.1(255–273)

His6-HvRST(467–579)

NBb

    

RST

AtDREB2A(244–272)

His6-GmRST(483–583)

320 ± 72

0.95 ± 0.02

−38.7 ± 1.0

1.6

−37.1

RST

ANAC013(254–274)

His6-GmRST(483–583)

526 ± 117

0.94 ± 0.02

−36.7 ± 1.1

0.8

−35.9

RST

GmDREB2A(273–290)

His6-GmRST(483–583)

2591 ± 515

0.98 ± 0.04

−23.5 ± 1.1

−8.4

−31.9

RST

GmDREB2A(273–290)

AtRST(499–572)

397 ± 34

0.85 ± 0.00

−34.9 ± 0.2

−1.6

−36.5

RST

  1. All experiments were performed as described in Methods. Syringe indicates whether RCD1–RST(499–572) or the TF is the titrant (in syringe). The standard errors for ΔH, Kd and N were obtained from Origin when fitting the data to a ‘one set of sites’ binding model. aPrevious data30. bNo detectable binding (NB).
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