Figure 3
From: Fundc1 is necessary for proper body axis formation during embryogenesis in zebrafish
Expression of genes related to body axis formation, autophagy, and apoptosis. (A) WISH of col2a1a, col8a1a, and shha in embryos at 24–28 hpf. (B) Expression of autophagy- and apoptosis-related genes in embryos at 24–28 hpf, detected using qRT-PCR. β-actin was used as an internal control. (C) WISH of cyclinD1, pax2a, opl, and neuroD1, which are involved in neural system development. (D) Gray-scale analysis of WISH results of genes in (C), whose signal strength was measured using ImageJ. (E) Expression of cyclinD1, pax2a, opl, and neuroD1 in embryos, detected using qRT-PCR, was normalized using β-actin.
