Table 1 Rates of abnormal embryos.

From: Fundc1 is necessary for proper body axis formation during embryogenesis in zebrafish

Experiment

Group

Total embryos

Abnormal embryos

Abnormal rate % (mean ± SE)

1

No injection

3146

55

1.75 ± 0.08 a

pSuperpuro

2742

103

3.75 ± 0.11 a

shRNAmis

1326

50

3.77 ± 0.35 a

shRNAran

1146

20

1.75 ± 0.02 a

shRNA1

3566

362

10.15 ± 0.30 b

shRNA2

7153

1264

17.67 ± 1.52 c

2

Control

2888

55

1.90 ± 0.09 a

shRNA2

3098

505

16.30 ± 0.58 b

shRNA2 + mRNA

2205

104

4.72 ± 0.31 c

  1. Note: Significant differences between groups are shown by different letters following the data (P < 0.05). The shRNAs – shRNA1, shRNA2 – target nucleotide 77–99 and 217–237 of Drfundc1 ORF respectively. shRNAmis, mismatched shRNA; shRNAran, random shRNA without any target gene.
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