Figure 8
From: Dynamics, nanomechanics and signal transduction in reelin repeats
AFM force spectroscopy captures unfolding of individual BEB domains and pre-burst state extension (as predicted by SMD simulations). The representative curves obtained with reelin samples (a,b) show characteristic tooth patterns, corresponding to sequential unfolding of BEB repeats. Solid lines show WLC model fits to the data (with a persistence length of 0.4 nm). Calculated contour length increment values are also shown. Some force extension curves (c) displayed a characteristic small (20 nm) plateau (marked with a green arrow) that appeared after the first nonspecific binding peak but preceded unfolding events. The appearance of this plateau corresponds well to SMD predicted small pre-burst extension of BEB domains.
