Figure 2 | Scientific Reports

Figure 2

From: Tuning the in vitro sensing and signaling properties of cyanobacterial PII protein by mutation of key residues

Figure 2The alternative text for this image may have been generated using AI.

Response of SeNAGK activity towards SePII (WT) or variants (K58N) and (K56N/L56K). (A) SeNAGK activity with or without different PII variants, as indicated. NAG was used as a variable substrate. (B) Arginine-feedback inhibition of SeNAGK activity in the presence or absence of different variants of PII protein, as indicated. Data were fitted according to a sigmoidal dose-response curve using GraphPad Prism to estimate the IC50 for arginine. (C) SeNAGK activity with or without different PII variants in presence of 11 µM arginine, as indicated. NAG was used as a variable substrate. (D) Effect of 2-OG on PII-promoted activation of SeNAGK in presence of 11 µM arginine, as indicated. SD as indicated by error bars, represents triplicate independent measurements.

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