Figure 5

Manhattan plot showing candidate gene associated with MTA identified for different traits and expression profile of candidate genes (CGs) during drought stress in Wheat-Exp database and quantitative real-time PCR (qRT-PCR) analysis. (a) TraesCS5B02G193100 (Trehalose-6-Phosphate; T-6-P), (b) TraesCS5B02G193200 (APETALA2/Ethylene-responsive factor; AP2/ERF TF), (c) TraesCS5A02G401800 (DNA-binding One Zinc Finger; Dof TF), (d) TraesCS2A02G547600 (Gibberellin-dioxygenases; GAox), TraesCS1B02G055800 (Histone-lysine N-methyltransferase; SET). 2−ΔΔCt method was used to calculate the relative expression levels of the target genes. Statistically significant differences between control and treated were calculated based on Student’s t-tests: *p < 0.01, NS, not significant. DTH_E2, days to heading in Meerut rainfed environment; PH_E2, plant height Meerut rainfed environment; DTM_E4, days to maturity Powerkheda rainfed environment; PH_E4, plant height Powerkheda rainfed environment. C, control; DS, drought stress, HS, heat stress.