Table 2 Michaels-Menten kinetic constants for WT-SPD in the absence and presence of 150 mM NaCl and/or 5 mM CaCl2 using the chromogenic substrate S-2288.

From: Characterization of the enzymatic activity of the serine protease domain of Factor VII activating protease (FSAP)

 

Buffer

Ca2+

Na+

Ca2+/Na+

kcat (min−1)

180 ± 122

743 ± 79

458 ± 77

707 ± 61

Km (µM)

1826 ± 796

146 ± 12

1275 ± 180

161 ± 27

kcat/Km (min−1 μM−1)

0.11 ± 0.1

5.1 ± 0.9

0.4 ± 0.06

4.4 ± 0.4

  1. The buffer used for these experiments was Tris (50 mM, pH 7.4) containing 2 mM EDTA and Tween-20 (0.1% wt/vol). All values are mean ± SD. Each value is obtained from 4–5 independent experiments.
Back to article page