Figure 5
From: I8-arachnotocin–an arthropod-derived G protein-biased ligand of the human vasopressin V2 receptor
Kinetics of OT/VP- and I8-arachnotocin-induced β-arrestin-2 recruitment at human OT/VP receptors. BRET was monitored between Nano-luciferase (Nluc) and EGFP introduced at the C-terminus of (a) OTR, (b) V1aR, (c) V1bR and (d) V2R (EGFP-OT/VP receptors) and the β-arrestin-2 (β-arrestin-2-Nluc). HEK293 cells co-expressing EGFP-OT/VP receptors and β-arrestin-2-Nluc were stimulated by 10 µM of VP, OT, and I8-arachnotocin respectively, 5 min after addition of the luciferase substrate (furimazine). The results are shown as differences in the BRET signals in the presence of ligands and are expressed as the mean value ± SD; n = 2.
