Figure 1 | Scientific Reports

Figure 1

From: High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice

Figure 1

Guide RNA design and in vitro cleavage analysis. (A) Rice phytoene desaturase gene (OsPDS) on chromosome 3. Dark green boxes represent exons, light green box and box arrow represents 5′ and 3′ untranslated regions, respectively. Two gRNA target sequences (in exon 1) are in blue and PAM sequences are in red. (B) In vitro cleavage assay for two target sequences PDS1 and PDS2. (C) In vitro cleavage assay for top two off-target sequences with highest homologies for PDS1 and PDS2. 2 m, 2 minutes; 30 m, 30 minutes; 3 h, 3 hours. a = target plasmid alone, b = target plasmid + gRNA 1 or 2, c = target plasmid + SpCas9, L = 1 kb DNA ladder.

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