Figure 3 | Scientific Reports

Figure 3

From: High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice

Figure 3

CRISPR-RNP delivery in rice. (A) Hygromycin resistant albino rice regenerants. (B) Hygromycin resistant green rice regenerants. (C) Both top and bottom gels represent agarose gel electrophoresis showing PDS amplicon, numbers represent independent transformation lines; 1 kb, molecular weight marker; wt, wild type; neg, water control. (D) Percentages of albino and green regenerants produced by three different transformation platforms. (E) Mutations in T0 transgenic lines. Blue letters, target sequences in PDS exon 1; Red letters, PAM sequences; White letters in blue or red boxes, substitution mutations; Black letter with underscore, insertion mutations; Event marked with star, event with random DNA inserted at the target sites; black vertical lines, positions where random DNA inserted; yellow highlighted numbers, sizes of random DNA fragments; WT, wild type; HM, homozygous; BI, biallelic; HT, heterozygous.

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