Table 1 Summary of on-target mutation of T0 rice transgenic plants generated using three different CRISPR reagent delivery platforms*.
Delivery method | Phenotype | # Hyg res lines | # Fertile | # Analyzed | # PDS mutated | Mutation typeƗ | # Random DNA insertion event | |||
|---|---|---|---|---|---|---|---|---|---|---|
Homozygous | Biallelic | Heterozygous | Mosaic | |||||||
RNP Cas9 + sgRNAs(1 + 2) (Biolistic) | Albino | 16 | 0 | 16 | 16 | 44% (7) | 56% (9) | 0% (0) | 0% (0) | 3 |
Green | 49 | 19 | 19 | 6 | 0% (0) | 0% (0) | 100% (6) | 0% (0) | 2 | |
pTF6005 (gRNA1) (Biolistic) | Albino | 3 | 0 | 3 | 3 | 67% (2) | 33% (1) | 0% (0) | 0% (0) | 1 |
Green | 32 | 10 | 10 | 3 | 0% (0) | 67% (2) | 33% (1) | 0% (0) | 0 | |
pTF6006 (gRNA2) (Biolistic) | Albino | 5 | 0 | 5 | 5 | 40% (2) | 60% (3) | 0% (0) | 0% (0) | 1 |
Green | 15 | 3 | 3 | 2 | 0% (0) | 0% (0) | 100% (3) | 0% (0) | 1 | |
pTF6005 (gRNA1) (Agro) | Albino | 4 | 0 | 4 | 4 | 25% (1) | 25% (1) | 0% (0) | 50% (2) | 0 |
Green | 2 | 1 | 1 | 1 | 0% (0) | 100% (1) | 0% (0) | 0% (0) | 0 | |
pTF6006 (gRNA2) (Agro) | Albino | 13 | 0 | 13 | 13 | 15% (2) | 85% (11) | 0% (0) | 0% (0) | 0 |
Green | 5 | 1 | 1 | 1 | 0% (0) | 100% (1) | 0% (0) | 0% (0) | 0 | |
