Figure 2
Screening of cross-reactive antigens against anti-HA antibody using CF-PA2Vtech (384-well plate version). (A) The images of the first screening using anti-HA antibody with the 384-well plate-based CF-PA2Vtech. In the first round of screening, using 12 plates of the 384-well plate version array in duplicate where 10 proteins are pooled per well, 12 spots were positive (red underlines). (B) The dot-blotting graph from the scan data of the first screening using anti-HA antibody. Average signal intensities of dual spots on the array image were plotted. On the graph, 12 positive spots showed higher signal intensities than the other spots (red colour characters). (C) The result images of second anti-HA antibody target screening using CF-PA2Vtech. The proteins in the positive result wells in the first round of screening were re-arrayed for individual testing on the array in duplicate. In the second round of screening, 13 proteins were recognized by the anti-HA-tag antibody. (D) The dot-blotting graph of the second anti-HA antibody target screening. Average signal intensities of dual spots of each protein on an array image were plotted. On the graph, 13 spots (red colour characters) showed higher signal intensities than the other spots and were determined as positive.
