Figure 3
From: Release of Porcine Sperm from Oviduct Cells is Stimulated by Progesterone and Requires CatSper
The effect of progesterone on sperm detachment from immobilized 3-O-sulfated Lewis X trisaccharide (suLeX). Sperm were allowed to bind to suLeX on beads for 45 min at 39 °C. Sperm bound to suLeX-beads were treated with 80 or 800 nM of progesterone or 80 nM, 17α-hydroxyprogesterone for 30 min at 39 °C. The number of sperm bound to beads was enumerated for at least 25 beads per droplet. Progesterone promoted a release of sperm from immobilized suLeX, but 17α-hydroxyprogesterone did not. This experiment was repeated three times. The asterisk represents a significant difference compared to 17α-hydroxyprogesterone (p < 0.05).
