Figure 4
From: Release of Porcine Sperm from Oviduct Cells is Stimulated by Progesterone and Requires CatSper
Progesterone stimulates Ca2+ influx in sperm. Fluo-4 loaded sperm were incubated in capacitating conditions at 39 °C with 80 nM of progesterone or vehicle. Spectrofluorimetric assessments corresponding to free intracellular Ca2 were recorded at 0, 15, and 30 min. Progesterone stimulated significantly more Ca2+ influx than the control at 30 min. This experiment was repeated three times. The asterisk represents a significant difference between treatments at the same time-point (p < 0.05).
