Figure 7
From: Release of Porcine Sperm from Oviduct Cells is Stimulated by Progesterone and Requires CatSper
Sperm release from oviduct cell aggregates induced by progesterone is dependent on CatSper channels. Sperm were allowed to bind to oviduct cell aggregates for 45 min at 39 °C and treated with 0 (vehicle control), 80 nM of progesterone, and 80 nM of progesterone plus 2 µM NNC 55-0396 for 30 min at 39 °C. The sperm-aggregate complexes were washed to remove loosely adherent sperm and transferred onto microscope slides to enumerate the number of sperm bound to the periphery of the aggregates. Progesterone reduced the number of sperm bound compared to vehicle control. Blocking CatSper channels abolished the effect of progesterone on sperm release. This experiment was repeated four times. An asterisk represents significant differences among different treatments (p < 0.05).
