Figure 8
From: Release of Porcine Sperm from Oviduct Cells is Stimulated by Progesterone and Requires CatSper
Sperm release from immobilized suLeX is dependent on CatSper channels. Sperm were allowed to bind to immobilized suLeX for 15 min at 39 °C and then treated with 0 (vehicle control), 80 nM of progesterone or 17α-hydroxyprogesterone, with or without 2 µM NNC 55-0396 for 30 min. Sperm bound to beads were enumerated. Progesterone reduced the number of sperm bound compared to vehicle control. Blocking CatSper channels abolished the effect of progesterone on sperm release. This experiment was repeated four times. The asterisk represents a significant difference compared to other treatments (p < 0.05).
