Figure 1 | Scientific Reports

Figure 1

From: Multi-parameter immune profiling of peripheral blood mononuclear cells by multiplexed single-cell mass cytometry in patients with early multiple sclerosis

Figure 1

Schematic representation of CyTOF measurement. Peripheral blood mononuclear cells (PBMCs) were collected from healthy controls (CON, n = 11) and patients with early multiple sclerosis (MS) (early MS, n = 11). PBMCs were CD45-barcoded and pooled. Mixed samples were equally divided and stained with two panels (Panel A and B, Supplementary Tables 2 and 3) of metal-conjugated antibodies and acquired on the CyTOF instrument. Prior to algorithm-based data analysis, the data were demultiplexed and compensated. Two steps of clustering analysis were performs; the primary supervised clustering analysis to identify the known lineage cell subsets, and the secondary unsupervised clustering analysis to discover small phenotypic differences within each identified lineage cell subset.

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