Figure 1

Oil Red O (ORO) staining in flight and ground mouse liver samples from RR-1 CASIS, RR-1 NASA and RR-3 missions. (A) Representative images of Oil Red O staining performed on OCT embedded frozen liver sections from flight and ground for all missions. Scale bar = 20 mm. (B) Flight samples from all missions show higher percent of tissue area stained with ORO indicative of liver damage across both strains of mice (unpaired Mann Whitney (Wilconox) test). (C) Temporal analysis of percent change of ORO lipid positivity in mice liver. Significant increase in lipid deposition is observed in livers of C57/BL6 exposed to microgravity for longer duration of time (unpaired Mann Whitney (Wilcoxon) test).