Figure 2 | Scientific Reports

Figure 2

From: Sideroflexin 4 affects Fe-S cluster biogenesis, iron metabolism, mitochondrial respiration and heme biosynthetic enzymes

Figure 2

SFXN4 knockout induces lactate secretion and triggers cell death in cells dependent on galactose as a carbon source. (A) Cell count measurements of K562 (Scramble/SFXN4 KO) cells cultured in glucose and galactose. (B) Percentage of cell death and caspase 3/7 activity in K562 (Scramble/SFXN4 KO) cells cultured in glucose and galactose. (C) BrDU incorporation in K562 (Scramble/SFXN4 KO) cells cultured in glucose and galactose. (D) Levels of lactate and extracellular acidification rate (ECAR) in the spent medium of K562 (Scramble/SFXN4 KO) cells. Shown are means and standard deviations of 4 replicas in a representative experiment of 3 independent experiments. (E) Immunoblot showing the levels of the glucose transporter GLUT1 and indicated glycolytic enzymes in K562 (Scramble/SFXN4 KO) cells.

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