Figure 3
An average increase in fluorescence of RBCs which presented anti-IgG Fc Alexa Fluor 647 fluorescence was presented by data collected via flow cytometry and in collected confocal microscopy images of live cells in media. (a) The average fluorescence of the control flow cytometry results was found. That value was then taken as a reference for the relative increase in fluorescence recorded by flow cytometry(n = 5). (b) Confocal microscopy images shown are from left to right accordingly, anti-CD235a fluorescence, anti-IgG Fc fluorescence, and an overlay of the two images to show binding presence across the RBC. (c) Confocal microscope images were further analyzed by measuring the average intensity above background noise. Fluorescent intensity is presented as mean fluorescence above the background of the image.
