Figure 1
From: SHBG141–161 Domain-Peptide Stimulates GPRC6A-Mediated Response in Leydig and β-Langerhans cell lines
(A) Lowest energy docked structures between extracellular domain of GPRC6A and respectively undercarboxylated osteocalcin (ucOCN, panel I), sex hormone binding globulin (SHBG, where only the interacting subunit of the dimer is shown, panel II) and SHBG(141–161)-domain peptide (SHBG141–161, panel III). (B) Estimated changes in free energy (ΔG) upon binding of GPRC6A with the aforementioned ligands ucOCN, SHBG and SHBG141–161. (C) Estimated conformation of the intracellular side of the transmembrane (TM) region of GPRC6A in its free form (Free, amber coiled structure in the inset) and upon the binding with SHBG141–161 (Bound, cyan coiled structure in the inset). (D) Estimated ΔG of the binding of GPRC6A with the Gαi(GDP) protein in its Free form and in complex with either ucOCN, SHBG and SHBG141–161.
