Figure 2
From: The bradykinin system in stress and anxiety in humans and mice
The effect of acute stress on bradykinin receptor protein expression in the hippocampus. We measured normalized protein expression levels of BDKRB1 and BDKRB2 using Western blot in hippocampus (a,b) and control stress-associated genes in hypothalamus (c–e) directly (2 h), 1 h (2 h + 1 h) or 5 h (2 h + 5 h) after 2 h restraint stress in C57BL/6NCrl mice. BRKRB1 HMW = bradykinin receptor B1 high molecular weight, BRKRB1 LMW = bradykinin receptor B1 low molecular weight, CRYAB = α-B-crystallin, FOS = FBJ osteosarcoma oncogene, HMGB1 = high mobility group B1. N = 3–5 in each experiment. Bars represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001compared to non-stressed control mice. For uncropped blots, see Supplemental Fig. 1.
