Figure 4

Vascular function. Male C57BL/6J mice were put on experimental diets at the age of 6 weeks for another 12 weeks. Vasodilation was induced by acetylcholine in norepinephrine-pre-contracted aorta in myograph experiments, without PVAT (A), with PVAT (B–E), or in the presence of NO synthase inhibitor L-NAME (E). *P < 0.05, **P < 0.01, ***P < 0.001. n = 9 (SCD and CAF) and 15 (NFD and HFD), respectively.