Figure 5
Proliferation (a,b,e,f) and osteogenic differentiation (c,d,g,h) of hMSC cultured in presence (indirect cell culture, a–d) and on the surface (direct cell culture, e–h) of B30, B30H20 and B30CK20. Attachment of hMSC on the surface of B30 (i), B30H20 (j) and B30CK20 (k), 24 h after seeding. Cells were seeded at 1.7 × 104 per 24-well and 2 × 104 per xerogel, respectively and were treated with 50 µM ascorbate on the first day of adherence (Os−; a,c,e,g). For osteogenic differentiation (Os+; b,d,f,h), hMSC were treated with 10 nM dexamethasone, 5 mM β-glycerophosphate and 50 µM ascorbate by day 3. Number of cells and ALP activity were measured from cell lysates by biochemical assays. *p < 0.05, **p < 0.01, ***p < 0.001. In fluorescence microscopic images, nuclei and F-actin were stained in blue and green, respectively.
