Figure 6
Validation of Fibronectin and Protein S100 A4 expression levels using immunoblot analysis. (A) Heat map showing the FN levels evaluated by mass spectrometry. (B) Quantification of FN levels from mass spectrometry were showed in a bar chart. (C) Immunoblot analysis of validating the FN levels in OGS freezing treated samples compared to OGS untreated and the data was normalized using beta actin. Full length immunoblots were showed in Fig. S1. (D) Bar chart showing the validation results of FN. (E) Heat map showing Protein S100 A4 levels evaluated by mass spectrometry. (F) Quantification of protein S100A4 levels from mass spectrometry were showed in a bar chart. (G) Immunoblot analysis validating Protein S100 A4 levels in OGS treated samples, data was normalized using beta actin. Full-length immunoblots were shown in Fig. S2. (H) Bar chart showing the quantification values of validation analysis.
