Figure 1 | Scientific Reports

Figure 1

From: [13C6,D8]2-deoxyglucose phosphorylation by hexokinase shows selectivity for the β-anomer

Figure 1The alternative text for this image may have been generated using AI.

13C-NMR spectra of [13C6,D8]2DG in a hyperpolarized state and at thermal equilibrium. (A) A 13C spectrum of hyperpolarized [13C6,D8]2DG recorded with a single scan using a 10° flip angle. (B) A spectrum of the same sample of [13C6,D8]2DG at thermal equilibrium acquired over 84 min using 64 excitations, 80 s repetition time, and the same flip angle (10°). The spectral processing for both spectra consisted of 10 Hz exponential line broadening and zero-filling from 8,192 to 16,834 points. Signal assignment: 1) C1β; 2) C1α, 3) C3, C4, and C5; 4) C6; 5) C2β; 6) C2α. The chemical shift scale was referenced to C6 of 2DG (signal 4) at 64.0 ppm25.

Back to article page