Figure 2
From: [13C6,D8]2-deoxyglucose phosphorylation by hexokinase shows selectivity for the β-anomer
Phosphorylation reactions of [13C6,D8]2DG by yHK and bGK. (A) A 13C spectrum showing all of the signals of hyperpolarized [13C6,D8]2DG and [13C6,D8]2DG6P during the reaction with yHK. (B) Consecutive 13C spectra of a typical experiment with yHK. The chemical shift regions of the C1, C6, and C2 signals of both [13C6,D8]2DG and [13C6,D8]2DG6P are presented. (C) The time course of the integrated signal intensities for the experiment shown in B for the signals centered at 66.59 (2DG6P C6), 94.69 (C1α), and 96.69 (C1β) ppm. (D) Consecutive 13C spectra of a typical experiment with bGK. The same chemical shift regions as in B are presented. (E) The time course of the integrated signal intensities for the experiment shown in D for the same chemical shift regions presented in C. (F) Consecutive 13C spectra of a typical experiment without an enzyme. The same chemical shift regions as in B are presented. (G) The time course of the integrated signal intensities for the experiment shown in F for the same chemical shift regions presented in C. All spectra were acquired over 50 s after the addition of hyperpolarized [13C6,D8]2DG, with a repetition time of 1 s and a 10° flip angle. yHK – yeast hexokinase, bGK - bacterial glucokinase, w/o - without, Intensity - integrated signal intensity, a.u. - arbitrary units.
