Figure 4
From: [13C6,D8]2-deoxyglucose phosphorylation by hexokinase shows selectivity for the β-anomer
Anomeric ratios over time in [13C6,D8]2DG and [13C6,D7]glucose reactions. The plots show the time course of the β/α ratio of the integrated signal intensities of the C1 positions in typical experiments. (A,B) Reactions of [13C6,D8]2DG and [13C6,D7]glucose, respectively, with yHK. (C,D) Reactions of [13C6,D8]2DG and [13C6,D7]glucose, respectively, with bGK, (no reaction for the former). (E,F) Experiments with [13C6,D8]2DG and [13C6,D7]glucose, respectively, without an enzyme. yHK – yeast hexokinase, bGK - bacterial glucokinase, w/o - without.
