Table 1 Enzymatic properties of recombinant PtomtAPX and PtosAPX.

From: PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr

APX isoforms

Substrate

Km (mM)

Vmax (mM min–1)

kcat (min–1)

kcat/Km (mM–1 min–1)

PtomtAPX

ASA

6.04 ± 0.06

3.00 ± 0.14

928405.63

153709.54

H2O2

0.03 ± 0.00

1.54 ± 0.00

475149.03

15838330.11

PtosAPX

ASA

4.30 ± 1.81

0.85 ± 0.25

897148.78

208505.07

H2O2

0.04 ± 0.01

1.64 ± 0.11

864716.73

20312959.95

  1. Values are means ± SDs of three replicates.