Figure 6

Effects of liver-specific Wdr76 overexpression in HFD-induced obese mice. (a) IB analyses were performed from epididymal WAT and liver extracts of Wdr76^+/+ and Wdr76^Li-TG mice to detect WDR76, HRas, PPARγ, C/EBPα and β-actin. Right panels show graphs for quantification of IB analyses (n = 3 per group). (b) Relative expressions of PPARγ, C/EBPα, and SREBP in epididymal WAT of Wdr76^+/+ and Wdr76^Li-TG mice (n = 5 per group). (c) Hepatic gene expression profile involved in metabolism from the liver of Wdr76^+/+ and Wdr76^Li-TG mice as determined by qRT-PCR (n = 5 per group). Expression was normalized to β-actin expression. Data are presented as the mean ± SD. Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.005.