Figure 1 | Scientific Reports

Figure 1

From: Molecular serotype-specific identification of Streptococcus pneumoniae using loop-mediated isothermal amplification

Figure 1

The relationship between reaction time and the absorbance of the reaction tubes. Colorimetric dye can be used with pyrophosphatase. Pyrophosphate, a by-product of the LAMP reaction, attenuates the activity of DNA polymerase. The addition of pyrophosphatase can increase the LAMP reaction speed. *, Bst DNA Polymerase (New England Biolabs, Ipswich, MS, USA) & conventional LAMP reagent; **, Isothermal Master Mix (no dye; Canon Medical Systems Corporation, Tochigi, Japan); PC, positive control; NC, negative control.

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