Table 2 Reactivity and specificity of the pneumococcal serotyping LAMP assay.

From: Molecular serotype-specific identification of Streptococcus pneumoniae using loop-mediated isothermal amplification

Vaccine

Serotypes

No. of strains

Strain ID

Origin of isolate

LAMP primer set

4

6B

9V

14

18C

19F

23F

1

3

5

7F

19A

PCV7

4

2

SP0852, SP0143

RTa, CSF

+b

6B

2

SP0857, SP1489

N, N

+

9V

2

SP0916, SP2928

N, B

+

14

2

SP0869, SP3320

N, B

+

18C

2

SP0873, SP2818

N, B

+

19F

2

SP0862, SP1118

N, N

+

23F

2

SP0885, SP2838

N, N

+

PCV13

1

2

SP3121, SP3070

B, B

+

3

2

SP1441, SP3256

N, N

+

5

2

SP3034, ATCC6305

U, U

+

6A

2

SP1567, SP1589

N, N

+

7F

2

SP3365, SP3172

B, RT

+

19A

2

SP1516, SP3081

N, N

+

PPSV23

2

1

D39

U

8

1

ATCC6308

U

9N

1

SP2700

CSF

10A

1

SP1933

N

11A/E

1

SP2760

RT

12F

1

SP0113

B

15B

1

SP3354

N

17F

1

NCTC11904

U

20

1

SP2830

N

22F

1

SP1854

N

33F

1

SP3201

N

Non- vaccine serotypes

6C

1

SP3362

N

+

6D

1

SP2739

N

+

7A

1

2040/37

U

+

7C

1

SP3285

N

9A

1

Wilder

U

+

13

1

SP0073

N

15A

1

SP2758

RT

15C

1

SP3343

N

18A

2

SP0852, 8609/43

B, U

+

18B

1

SP1901

N

+

18F

1

Lederle

U

+

23A

1

SP3374

N

24F

1

SP3193

N

34

1

SP3359

N

35B

1

SP3357

N

37

1

SP2742

N

38

1

SP3356

N

  1. aRT, respiratory tract specimen; CSF, cerebrospinal fluid; N, nasopharyngeal swab; B, blood; U, unknown.
  2. b+, Amplification within 25 min incubation; −, no amplification within 120 min incubation.