Table 3 Detection limits of LAMP and PCR assays detecting DNAs of pneumococcal serotypes and using the DNA spiked CSF specimens.

From: Molecular serotype-specific identification of Streptococcus pneumoniae using loop-mediated isothermal amplification

S.pneumoniae serotypes

Detection limit (Purified DNA)

Detection limit (DNA spiked CSFa)

Detection limit (DNA spiked blood)

PCRb,c

LAMPb

PCR

LAMP

PCR

LAMP

4

102copies d

102

102

102

>105

102

6B

102

102

103

102

105

102

9V

102

102

102

102

>105

102

14

102

10

103

10

105

10

18C

102

10

102

10

>105

102

19F

10

10

10

10

103

10

23F

102

10

103

10

>105

102

1

103

102

103

102

>105

102

3

103

102

103

102

105

102

5

104

102

104

102

>105

102

7F

102

102

102

102

>105

102

19A

102

102

102

102

>105

102

  1. aCerebrospinal fluid specimen collected between 1998 and 200239.
  2. bPCR results were obtained by electrophoretic analysis. LAMP results were determined visually.
  3. cconventional PCR (serotypes 1, 3, 4, 5, 6B, 18C, 19F and 23F11; serotypes 9V, 7F12; serotype 1414; serotype 19A13).
  4. dNumber of genome copies per reaction.
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