Figure 7
From: MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus
Northern blot for mitosRNA-tRNA-cys 3′ variants in cells treated with ethidum bromide or actinomycin to inhibit mitochondrial and nuclear transcription, respectively. Cells were pre-treated with ethidium bromide (0.4 µg/ml) to inhibit mitochondrial transcription or with actinomycin (10 µM) to inhibit nuclear transcription for 3 h prior to 24 h of anoxia or normoxia. Actinomycin-treated cells also received 0.1% of DMSO and thus cells treated with 0.1% DMSO were sampled as a control. “N” indicates normoxia and “A” indicates 24 h of anoxia. 20 µg RNA was loaded for each sample and the blot was hybridized with 2 nM mitosRNA-tRNA-cys probe.
