Figure 5
Metabolic activity measurements of bioluminescent Escherichia coli Xen14 treated with sanitizers added to artificially-contaminated water. Bacteria were treated with electrolyzed oxidizing water (EOW), sodium hypochlorite (NaClO) or chlorine dioxide (ClO2) prepared at 0, 1, 5, 20 and 50 mg/L free chlorine in the presence of either 40 mg/L dissolved organic carbon (DOC) from Suwannee river natural organic matter (SRNOM) or 100 mg/L DOC from cow manure for 120 s. Untreated bacteria resuspended in sterile water, 40 mg/L DOC from SRNOM or 100 mg/L DOC from cow manure were used as controls. Aliquots of treated samples were added to 200 µL sterile Luria Bertani broth and then incubated at 37 °C in a Cytation 5 Cell Imaging Multi-Mode Reader. Total luminescent signals (relative light units) were collected over a 40 h incubation period. Figures were generated using Prism v8 (GraphPad Software, San Diego, CA, USA).
